By Wayne W Umbreit
Meant for researchers in utilized microbiology and environmental engineers, this e-book covers such subject matters as environmental overview of biotechnological procedures and microbial changes of haloaromatic and haloaliphatic compounds.
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Extra resources for Advances in Applied Microbiology, Vol. 3
A 2-liter con- PRESERVATION OF BACTERIA BY LYOPHILIZATION 19 denser which can lyophilize up to 800 ml. at a time can be cooled in a 1-gallon thermos jar. ) in a single flask but a manifold could be attached to permit the use of multiple chambers. A more complex arrangement described by Stull and Herreid (1949) included a mercury diffusion pump and certainly required the services of an expert glass blower to assemble it. On the basis of the data presented the performance of their apparatus did not seem to be significantly superior to that of other simpler designs.
One essential factor pointed out by Hornibrook (1949) is that the acid used to absorb moisture must be kept clean and especially free of salt, since liberated hydrochloric acid can be very toxic in the closed system. The modified Holzman (1950) apparatus described by Pratt and Yamaguchi (1950) is another relatively easily constructed glass lyophil apparatus of the manifold type. ” The condenser was an 800-ml. Kjeldahl flask immersed in dry ice and attached to the joint pointing downward. Manifolds or other flasks with material to be dried were attached to the horizontal joints, The system was evacuated through a 10-mm.
The culture is at the maximum stationary phase. C. CELLCONCENTRATION The highest concentration of cells which can be conveniently prepared and dispensed is ideal since the objective is to obtain dry cells and any unnecessary water is avoided. , 1958 and others). Fry (1954) stated that when cultures are dried with adequate protective colloid the percentage survival probably is independent of the initial cell concentration. However, it is not easy to be certain that the protective colloid is adequate and hence the cell concentration may indeed be important.
Advances in Applied Microbiology, Vol. 3 by Wayne W Umbreit